Rabbit Monoclonal IgG Anti-AMACR (P504S) clone 13H4 [13H4]

Basic information

  • Name

    Rabbit Monoclonal IgG Anti-AMACR (P504S) clone 13H4 [13H4]

  • Price

    841 EUR

  • Size

    1 ml

  • Catalog number

    P504S-1.0

More detailed information

Availability

Please contact us to check the availability

Immunogen

Human recombinant AMACR (P504S) protein, whole sequence

Specificity

Human AMACR (P504S) protein, Alpha-Methylacyl CoA Racemase

Species reactivity

Human

Product presentation

Ion exchange chromatography purified antibody in PBS, BSA, sodium azide (0.09%)*. Use antibody dilution buffer (e.g. Art. No. PU002) containing sufficient protein and preservative.

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

13H4

Product is supplied as

liquid concentrate

Recommended concentration for use

1:80 – 1:200

Protein concentration

40 µg/ml

Positive control

Prostate carcinoma

Pretreatment

In formaldehyde fixed tissue unmasking of antigen by heat pre-treatment is required. We recommend the application of our unmasking fluid G on glycol basis (Art. No. DE007) or EDTA solution pH 9 (Art.-No. DE006) approx. 30 min in pressure cooker or micro wave oven. Citrate buffer is also suitable, but gives less intensity in staining and changes the cell morphology more than the above mentioned unmasking methods.

Tested applications

IHC(C, P)

Recommended secondary reagent

Any secondary system containing anti-rabbit IgG may be used. Best results have been generated with DAKO Envision Plus, VECTOR ImmPress Rabbit IgG (MP-7401) and VECTASTAIN ABC Systems (Art. No. PK-6101 or AK-5001) in combination with a suitable substrate.

Storage method

Store at 2-8°C

UniProt number

Q9UHK6

Litterature

1. Schmitz W., Albers C., Fingerhut R., and Conzelmann E. (1995) Purification and characterization of an alpha-methylacyl-CoA racemase from human liver. Eur J. Biochem. 231(3); 815-822. 2. Babcook J.S., Leslie K.B., Olsen O.A., et al. (1996) A novel strategy for generating monoclonal antibodies from single isolated lymphocytes producing antibodies of defined specificities. Proc. Natl. Acad. Sci. USA 93; 7843-7848. 3. Xu J., Stolk J.A., Zhang X., et al. (2000) Identificatin of differentially expressed genes in human prostate cancer using subtraction and microarray. Cancer Res. 60; 1677-1682. 4. Ferdinandusse S., Denis S., IJ1st, et al. (2000) Subcellular localization and physiological role of alpha-methylacyl-coA racemase. J. Lipid Res. 41; 1890-1896. 5. Jiang Z., Woda B.A., Rock K.L., Xu Y., Savas L., et al. (2001) P504S a new molecular marker for the detection of prostate carcinoma. Am. J. Surg. Pathol. 25(11): 1397-1404.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus