Rabbit Monoclonal IgG Anti-Synaptophysin, SYP (SP11) [SP11]

Basic information

  • Name

    Rabbit Monoclonal IgG Anti-Synaptophysin, SYP (SP11) [SP11]

  • Price

    436 EUR

  • Size

    0.5 ml

  • Catalog number

    SYP011-0.5

More detailed information

Availability

Please contact us to check the availability

Immunogen

Synthetic peptide corresponding to the C-terminus of human Synaptophysin

Specificity

Synaptophysin, 38 kDa protein in neurectodermal tissue

Species reactivity

Human, cattle, mouse, rat

Product presentation

Tissue culture supernatant containing 0.2% BSA and 15 mM sodium azide**

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

SP11

Product is supplied as

liquid concentrate

Recommended concentration for use

1:50-1:100

Protein concentration

N/A

Positive control

Adrenal medulla, brain, appendix

Pretreatment

Pre-treatment with unmasking fluids G (Art. No. DE007) or C (Art. No. DE000).

Tested applications

IHC(P)

Recommended secondary reagent

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable as well as polymeric systems as long as they contain anti-rabbit conjugates.

Storage method

Store at 2-8°C

UniProt number

PGo to UniProt/SwissProt8247 (SYPH_HUMAN)

Litterature

1. Wiedenmann B. and Franke W.W. (1985) Identification and localization of synaptophysin, an integral membrane glykoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 41; 1017-1028. 2. Wiedenmann B., Franke W.W., Kuhn C., Moll R., Gould V.E. (1986) Synaptophysin a marker protein for neuroendocrine cells and neoplasms. Proc. Natl. Acad. Sci (USA) 83; 3500-3504. 3. Buffa R., Rindi G., Sessa F., Gini A., Capella C., Jahn R., et al. (1988) Synaptophysin immunoreactivity and small clear vesicles in neuroendocrine cells and related tumours. Moel. Cellul. Probes 2; 367-381. 4. Stefaneanu L., Ryan N, Kovacs K. (1988) Immunocytochemical localization of synaptophysin in human hypophysis and pituitary adenomas. Arch. Pathol. Lab. Med. 112; 801-804

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus