Chicken anti Rat IgG H + L biotin
241 EUR
500 µg
43R-1511
Secondary Antibody
Conjugated Secondary Antibodies
Immunology
Purified Rat IgG (H&L)
Chicken
This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.
No reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or rabbit or serum proteins from human or rabbit.
Biotin
Grater than 95% pure
Affinity purified using solid phase Rat IgG (H&L)
Ig fraction was prepared using eggs obtained from healthy hens of US origin.
1.0 mg/ml (lot specific)
Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
Store at 2-8 deg C.
Blue Ice
Please refer to the datasheet
1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
Biotinylated
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
IgG
Rattus norvegicus