Donkey anti Rabbit IgG H + L HRP

Basic information

  • Name

    Donkey anti Rabbit IgG H + L HRP

  • Price

    352 EUR

  • Size

    1 mg

  • Catalog number

    43R-ID043hrp

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Donkey anti-Rabbit IgG (H + L) (HRP) was raised in donkey using purified Rabbit IgG (H&L) ..

Raised in

Donkey

Specificity

This antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins

Cross Reactivity

No reactivity is observed to non-immunoglobulin rabbit serum proteins or IgG from bovine, chicken, Goat, Guinea Pig, hamster, horse, human, mouse, rat or sheep.

Conjugate/Tag

HRP

Method of Purification

Affinity purified using solid phase Rabbit IgG (H&L)

Source

Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Form & Buffer

Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.1% (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 1.1 ml of deionized water. Prepare fresh working dilution daily.

Storage

Store lyophilized material at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

Please refer to the datasheet

Usage Recommendations

1:200-1:5000 ELISA/WB, 1:500-1:5000 IHC

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Equus africanus asinus,Oryctolagus cuniculus

Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Conjugation

HRP Conjugated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG