Donkey anti Rat IgG H + L HRP

Basic information

  • Name

    Donkey anti Rat IgG H + L HRP

  • Price

    204 EUR

  • Size

    1 mg

  • Catalog number

    43R-1519

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Rat IgG (H&L)

Raised in

Donkey

Specificity

This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.

Cross Reactivity

No reactivity is observed to non-immunoglobulin rat serum proteins.

Conjugate/Tag

HRP

Grade & Purity

Grater than 95% pure

Method of Purification

Affinity purified using solid phase Rat IgG (H&L)

Source

Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Concentration

1.0 mg/ml (lot specific)

Form & Buffer

Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.1% (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 1.1 ml of deionized water. Prepare fresh working dilution daily.

Storage

Store lyophilized material at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

Please refer to the datasheet

Usage Recommendations

1:200-1:5000 ELISA/WB, 1:500-1:5000 IHC

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Equus africanus asinus,Rattus norvegicus

Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Conjugation

HRP Conjugated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Additional isotype

IgG