Rabbit anti Rat IgG H + L rhodamine

Basic information

  • Name

    Rabbit anti Rat IgG H + L rhodamine

  • Price

    204 EUR

  • Size

    1 mg

  • Catalog number

    43R-1555

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Rat IgG (H&L)

Raised in

Rabbit

Specificity

This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.

Cross Reactivity

No reactivity is observed to non-immunoglobulin rat serum proteins.

Conjugate/Tag

Rhodamine

Grade & Purity

Grater than 95% pure

Method of Purification

Affinity purified using solid phase Rat IgG (H&L)

Source

Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Concentration

1.0 mg/ml (lot specific)

Form & Buffer

Lyophilized from 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2. Dilute in dH2O to a concentration of 1 mg/ml

Storage

Store at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

FC; IF; IHC

Usage Recommendations

The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus,Rattus norvegicus