Donkey anti Goat IgG H + L Alexa Fluor 647

Basic information

  • Name

    Donkey anti Goat IgG H + L Alexa Fluor 647

  • Price

    414 EUR

  • Size

    500 µg

  • Catalog number

    43R-ID028AF

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Raised in

Donkey

Specificity

Goat IgG

Cross Reactivity

Minimal cross reaction with Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat

Isotypes

IgG

Conjugate/Tag

Alexa Fluor 647

Method of Purification

Donkey anti Goat IgG (H + L) (Alexa Fluor 647) was purified by affinity chromatography.

Concentration

1.4 mg/ml

Form & Buffer

Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.

Storage

Store at 2-8 deg C until reconstitution. Following reconstitution aliquot and freeze at -70 deg C for long term storage. Avoid repeated freeze-thaw cycles.

Shipping conditions

Blue Ice

Tested for

Please refer to the datasheet

Usage Recommendations

1:100 - 1:800 for most applications.

Additional Information

Alexa Fluor 647 Carboxylic Acid, Amax=651; Emax=667. Absorbance Ratio: A651/A280 = 4.3

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. fitzgerald made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the anti Goat IgG H + L exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.

Conjugation

Alexa Fluor,ALEXA FLUOR 647

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Equus africanus asinus,Capra aegagrus hircus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG