Donkey anti Rat IgG H + L Fab'2 biotin

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Basic information

  • Name

    Donkey anti Rat IgG H + L Fab'2 biotin

  • Price

    389 EUR

  • Size

    300 µl

  • Catalog number

    43R-ID029bt

  • Buy online

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Donkey anti-rat IgG (H + L) (Fab'2) (biotin) was raised in donkey using rat IgG (H&L) ..

Raised in

Donkey

Specificity

Rat

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

Biotin

Method of Purification

Donkey anti Rat IgG (H + L) (Fab'2) (biotin) was purified by affinity chromatography.

Form & Buffer

Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.

Storage

Store at 4 deg C.

Shipping conditions

Blue Ice

Tested for

ELISA; FC; ICC; IHC; WB

Usage Recommendations

ELISA: 1:20,000-1:400,000, FC: 1:200-1:1,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, WB: 1:20,000-1:400,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent

Conjugation

Biotinylated

Latin name

Equus africanus asinus,Rattus norvegicus

Additional isotype

 Fab fragment,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.