Donkey anti Sheep IgG H + L biotin

Basic information

  • Name

    Donkey anti Sheep IgG H + L biotin

  • Price

    290 EUR

  • Size

    500 µl

  • Catalog number

    43R-ID025bt

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Donkey anti-sheep IgG (H + L) (biotin) was raised in donkey using sheep IgG (H&L) ..

Raised in

Donkey

Specificity

Sheep

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

Biotin

Method of Purification

Donkey anti Sheep IgG (H + L) (bioton) was purified by affinity chromatography.

Form & Buffer

Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3. Reconstitute with 0.5 ml of distilled Water.

Storage

Store at 4 deg C.

Shipping conditions

Blue Ice

Tested for

ELISA; FC; ICC; IHC; WB

Usage Recommendations

ELISA: 1:20,000-1:400,000, FC: 1:200-1:1,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, WB: 1:20,000-1:400,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Latin name

Equus africanus asinus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG