Rabbit anti Dog IgG H + L biotin

Basic information

  • Name

    Rabbit anti Dog IgG H + L biotin

  • Price

    290 EUR

  • Size

    1.5 mg

  • Catalog number

    43R-IR003bt

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Rabbit anti-canine IgG (H + L) (biotin) was raised in rabbit using canine IgG (H&L) ..

Raised in

Rabbit

Specificity

Dog

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

Biotin

Method of Purification

Rabbit anti Dog IgG (H + L) (biotin) was purified by affinity chromatography.

Form & Buffer

Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3. Reconstitute with 1.5 ml of distilled water.

Storage

Store at 4 deg C.

Shipping conditions

Blue Ice

Tested for

ELISA; FC; ICC; IHC; WB

Usage Recommendations

ELISA: 1:20,000-1:400,000, FC: 1:200-1:1,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, WB: 1:20,000-1:400,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Canine or Canis Lupus is mostly Beagle used for drug research. fitzgerald produces ELISA test kits and polyclonal antibodies.

Conjugation

Biotinylated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus