Biotin-Linked Rabbit Anti-Feline IgG
A secondary Biotin-Linked Rabbit Anti-Feline IgG polyclonal antibody for WB; IP; IHC-P; IHC-F; ELISA.
Type: Secondary antibody; Host: Felis catus; Feline (Cat); Species reactivity: Rabbit; Clonality: Polyclonal; Conjugation: Biotin; Antigen: Feline IgG ; Concentration: 500ug/ml; Isotype: Immunoglobulin G; Applications:WB; IP; IHC-P; IHC-F; ELISA.
Buffer composition may vary and may be adjusted per customer's request.
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
Research Use Only.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by Cloud Clone Corp. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cloud Clone Corp adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.