chicken polyclonal anti-Mouse IgG (Fab)2-HRP mammalian protein antibody

Basic information

  • Name

    chicken polyclonal anti-Mouse IgG (Fab)2-HRP mammalian protein antibody

  • Price

    238 EUR

  • Size

    500 ug

  • Catalog number

    AMIGFab-HRP

More detailed information

Antigen

Mouse IgG (Fab)2-HRP

Chicken IgY

Gallus gallus domesticus and other IgY antibodies have high affinity and are easy to collect from the yolk. Gallus gallus can produce 20 more than in rabbit polyclonal. The antibody will not bind to rheumatoid factor or protein or Fc regions. This gives very much reduced background very much suited for diagnostic applications. Papain will digest IgY’s to divalent Fab antibody fragments. IgY’s are suited for Elisa kit development, Immunohistochemistry and Western Blot.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by gallus IgY they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent,HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Additional isotype

 Fab fragment,IgG

Conjugation

HRP Conjugated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus

Group

Polyclonals and antibodies

French translation

anticorps