FITC Conjugated Goat Anti-rabbit IgG antibody

Basic information

  • Name

    FITC Conjugated Goat Anti-rabbit IgG antibody

  • Price

    158 EUR

  • Size

    500 µl

  • Catalog number

    BA1105-0.5

More detailed information

Type of the antibody/protein

IgG secondary antibody

Raised in/synthesized

Goat

Conjugation

FITC

Product configuration

Solution with concentration 1mg/ml

Immunogen

Rabbit IgG (whole molecule)

Clonality

Polyclonal antibodies

Analyses

IHC-P,IHC-F,ICC,FCM

Storage conditions

Keep the FITC Conjugated Goat Anti-rabbit IgG antibody at plus four degrees Celsius for 12 months.

Product details

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations.

Specificity

This FITC conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG.

Tips

The FITC Conjugated Goat Anti-rabbit IgG antibody is intended to be used for research analyses and it is not applicale for in vitro diagnostics.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This boster Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. boster FITC is highly sensitive to pH extremes.

Additional conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus,Oryctolagus cuniculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by boster. Rabbit antibodies are very stable and can be stored for several days at room temperature. boster adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

French translation

anticorps