Cy3 Conjugated anti-Mouse IgG SABC Kit

Basic information

  • Name

    Cy3 Conjugated anti-Mouse IgG SABC Kit

  • Price

    450 EUR

  • Size

    1 kit

  • Catalog number

    SA1072

More detailed information

Immunogen

NA

Storage & Transport Conditions

4°C for one year. Avoid freezing

Kit Components

1. Normal goat serum blocking reagent: 10x, 5 ml, for the block of tissue sections. 2. Biotinylated Secondary Antibody (Goat Anti-mouse IgG): 100x, 0.5 ml (2mg/ml). Affinity purified antibody, labeled with “long-armâ€ biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester, CAS# 72040-63-2). 3. SABC-Cy3 (Cy3 conjugated streptavidin): 100x, 0.5ml (1mg/ml). Manufactured by Boster’s proprietary method, the complex is very stable and offers superior amplification of the antigen signals. 4. Three drop bottles (For dilution use).

Applications

IHC, ICC

Reactivity

NA

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Conjugated anti-Mouse IgG SABC Kit can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This boster antibody is well suited for fluorescent microscopy.

Conjugation

cy3 conjugation kit

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus