Rabbit Anti-Mouse IgG-Fc Antibody, Biotin Conjugated

Basic information

  • Name

    Rabbit Anti-Mouse IgG-Fc Antibody, Biotin Conjugated

  • Price

    121 EUR

  • Size

    0.2ml

  • Catalog number

    bs-0377R-Biotin

More detailed information

Category

Conjugated Secondary Antibody

Conjugation

Biotin

Host Organism

Rabbit

Target antigen

Mouse IgG

Modification

Unmodified

Modification site

None

Clonality

Polyclonal antibody

Clone

Polyclonal antibody

Concentration

1ug/ul

Tested applications

WB, ELISA, IHC-P, IHC-F

Recommended dilutions

WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)

Cross reactivity

Mouse

Background of the target antigen

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Purification method

This antibody has been purified via Protein A.

Storage conditions

Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Additional conjugation

Biotinylated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by Bioss Secondary Antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioss Secondary Antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus,Oryctolagus cuniculus

French translation

anticorps