Anti-Mouse alpha/beta TCR, Biotin (Clone H57-597) (hamster IgG)

Basic information

  • Name

    Anti-Mouse alpha/beta TCR, Biotin (Clone H57-597) (hamster IgG)

  • Price

    260 EUR

  • Size

    100ug

  • Catalog number

    GEN520006

More detailed information

Also known as

alpha/beta TCR

Other names

N/A

Category

Antibodies

Subcategory

Mnoclonal antibodies

Gene name

alpha/beta TCR

Gene name synonims

N/A

Other gene names

N/A

Clonality

Monoclonal

Immunoglobulin isotype

Hamster IgG

Clone

H57-597

Host organism

Hamster

Species reactivity

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Specificity and cross-reactivity

Alpha/Beta TCR (Mouse ab TCR); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Purification method

N/A

Form/Appearance

Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))

Concentration

Antibody Concentration: 0.1 miligram per 1 mililiter

Storage and shipping

Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles

Tested applications:

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Description

The Anti-Mouse alpha/beta TCR, (Clone H57-597) ( IgG) is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.

Conjugation

Biotinylated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus