Mouse anti-RBM3 (IgG), monoclonal, clone Ahuman9

Basic information

  • Name

    Mouse anti-RBM3 (IgG), monoclonal, clone Ahuman9

  • Price

    890 EUR

  • Size

    1 mg lyophilized

  • Catalog number

    12 100 010

More detailed information

Antibody category

Monoclonal Antibody

Antibody's description

Monoclonal antibody to human RBM3 (RNA-binding motive 3). Immunoglobulin isotype: IgG1. The monoclonal antibody was raised against recombinant human RBM3. The immune lymphocytes from BALB/c mice were extracted from lymph nodes and fused with myeloma sp2/0 cells according to a standard protocol. The IgG1 fraction was purified by Protein G affinity chromatography.

Raised in

mouse

Research area of interest

Neurodegenerative diseases, Cardiac diseases, Cancer, Therapeutic Hyperthermia

Package form

lyophilized

Clonality

Monoclonal

Antigen

recomb. human RBM3

Reacts with

Human

Immunogen

See the data sheet or contact us

Contents

PBS, with 0,1% Azide

Purity

>95% pure. Purity is tested by electrophoresis.

Tested applications

• ELISA, • Flow Cytometry • Immunoblotting • Immunofluorescence • Immunohistochemistry • Immunoprecipitation ,

Other names

Mab to human RBM3 (RNA-binding motive 3). Immunoglobulin isotype: IgG1. The monoclonal antibody was raised against recombinant human RBM3. The immune lymphocytes from BALB/c mice were extracted from lymph nodes and fused with myeloma sp2/0 cells according to a standard protocol. The IgG1 fraction was purified by Protein G affinity chromatography.

Crossreactivity

no crossreactivty to human Cold inducible Binding protein (CIRBP)

Antibody specificity

recomb. and human RBM3

Purification

Protein A purified

PubMed citations

Arrica, M. & Bissonnette, B. (2007). Semin Cardiothorac Vasc Anesth. 11, 6–15. Bernard, S. A., Gray, T. W., Buist, M. D., Jones, B. M., Silvester, W., Gutteridge, G. & Smith, K. (2002). N. Engl. J. Med. 346, 557–563. Chip, S., Zelmer, A., Ogunshola, O. O., Felderhoff-Mueser, U., Nitsch, C., Bührer, C. & Wellmann, S. (2011). Neurobiol. Dis. 43, 388–396. Dresios, J., Aschrafi, A., Owens, G. C., Vanderklish, P. W., Edelman, G. M. & Mauro, V. P. (2005). Proc. Natl. Acad. Sci. U.S.A. 102, 1865–1870. Groysman, L. I., Emanuel, B. A., Kim-Tenser, M. A., Sung, G. Y. & Mack, W. J. (2011). Neurosurg Focus. 30, E17. Peretti, D., Bastide, A., Radford, H., Verity, N., Molloy, C., Martin, M. G., Moreno, J. A., Steinert, J. R., Smith, T., Dinsdale, D., Willis, A. E. & Mallucci, G. R. (2015). Nature. 518, 236–239. Shankaran, S., Laptook, A. R., Ehrenkranz, R. A., Tyson, J. E., McDonald, S. A., Donovan, E. F., Fanaroff, A. A., Poole, W. K., Wright, L. L., Higgins, R. D., Finer, N. N., Carlo, W. A., Duara, S., Oh, W., Cotten, C. M., Stevenson, D. K., Stoll, B. J., Lemons, J. A., Guillet, R., Jobe, A. H. & National Institute of Child Health and Human Development Neonatal Research Network (2005). N. Engl. J. Med. 353, 1574–1584.

UniProt number

P98179

Storage conditions

-20°C long term; +4°C short term

Technical datasheet

Contact us to receive the datasheet

Shipping condition

-80°C +37°C

Warnings

For Research Use only.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus