Mouse anti-TNF (IgG), monoclonal

Basic information

  • Name

    Mouse anti-TNF (IgG), monoclonal

  • Price

    890 EUR

  • Size

    1 mg lyophilized

  • Catalog number

    12 500 010

More detailed information

Antibody category

Monoclonal Antibody

Antibody's description

Monoclonal antibody to human TNF. Immunoglobulin isotype: IgG2a. The monoclonal antibody was raised against recombinant human tumor necrose factor alpha (TNFa). The immune lymphocytes from BALB/c mice were extracted from lymph nodes and fused with myeloma sp2/0 cells according to a standard protocol. The IgG1 fraction was purified by Protein G affinity chromatography. The antibody has been reported for use in detection of human TNF alpha by ELISA. It can also be used for immunoblotting.

Raised in

mouse

Research area of interest

Autoimmune disease

Package form

lyophilized

Clonality

Monoclonal

Antigen

recombinant human TNF

Reacts with

Human

Immunogen

See the data sheet or contact us

Contents

PBS, with 0,1% Azide

Purity

>95% pure. Purity is tested by electrophoresis.

Tested applications

• ELISA • Immunoblotting

Other names

Mab to human TNF. Immunoglobulin isotype: IgG2a. The monoclonal antibody was raised against recombinant human tumor necrose factor alpha (TNFa). The immune lymphocytes from BALB/c mice were extracted from lymph nodes and fused with myeloma sp2/0 cells according to a standard protocol. The IgG1 fraction was purified by Protein G affinity chromatography. The antibody has been reported for use in detection of human TNF alpha by ELISA. It can also be used for immunoblotting.

Crossreactivity

no crossreactivty

Antibody specificity

recombinant and human TNF

Purification

Protein G purified

PubMed citations

1. Strohner P: Patent: EP 1957980B1 Immunoassay for the simultaneous immunochemical determination of an analyte (antigen) and a treatment antibody targeting the analyte in samples (Recovery immunoassay) 2. Korn S, Haasler I, Jung M, Strohner P, Staatz A, Schäfer A, Becher G, Taube C and Buhl R Free serum IgE in patients with severe allergic asthma treated with Omalizumab Am. J. Respir. Crit. Care Med., May 2010; 181: A6655 3. Strohner P, Sarrach D, Reich JG, Becher G, Staatz A, Schäfer A, Steiß J-O, Häupl T: The Recovery-ELISA – a novel Assay Technique for the Therapy Control of Therapeutic Antibodies Am J Respir Crit Care Med 181;2010:A5674 4. Strohner P, Staatz A, Schäfer A, Becher G, Sarrach D, Reich JG, Steiß J-O, Häupl T: Monitoring of Monoclonal Antibody Therapy with a new Recovery ELISA Assay Technique (R-ELISA®) ERS Barcelona 2010, Abstract-No: 3399 5. Steiß JO, Schmidt A, Lindemann H, Rudloff S, Staatz A, Strohner P, Becher G, Nährlich L, Zimmer KP: Therapiemonitoring von Omalizumab im Kindes- und Jugendalter. Allergologie, Jahrgang 33, Nr. 10/2010, S. 421–428

UniProt number

P01377

Storage conditions

-20°C long term; +4°C short term

Technical datasheet

Contact us to receive the datasheet

Shipping condition

-80°C +37°C

Warnings

For Research Use only.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus

Gene

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.